•F1000Research
A draft reference assembly of the Psilocybe cubensis genome
open
Chin, C-S., Crawford, S., Kane, L. T., McKernan, K., McLaughlin, S., Trippe, A.
This genomic archive paper (2021) contains the entire genetic sequence of the Psilocybe cubensis mushroom, more specifically the genome cluster for psilocybin synthesis.
Abstract
We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster exists in a single 3.2Mb contig. The dataset is available from NCBI BioProject with accessions PRJNA687911 and PRJNA700437.