Trial PaperDepressive DisordersMajor Depressive Disorder (MDD)Treatment-Resistant Depression (TRD)Immunology & InflammationAyahuasca

Changes in inflammatory biomarkers are related to the antidepressant effects of Ayahuasca

In a double‑blind placebo‑controlled trial of treatment‑resistant depression patients and healthy controls, ayahuasca produced a significant 48‑hour reduction in C‑reactive protein (CRP) and, in patients, larger CRP decreases correlated with greater reductions in depressive symptoms. No significant effects were seen for interleukin‑6 or BDNF, and these biomarkers did not predict antidepressant response or remission.

Authors

  • Fernanda Palhano-Fontes
  • Nathalia Galvão-Coelho
  • Daniel Perkins

Published

Journal of Psychopharmacology
individual Study

Abstract

Background

Ayahuasca is a traditional Amazon brew and its potential antidepressant properties have recently been explored in scientific settings. We conducted a double-blind placebo-controlled trial of ayahuasca with treatment-resistant depression patients ( n = 28) and healthy controls ( n = 45).

Aims

We are evaluating the blood inflammatory biomarkers: C-reactive protein and interleukin 6, as a potential consequence of ayahuasca intake and their correlation with serum cortisol and brain-derived neurotrophic factor levels. Blood samples were collected at pre-treatment and 48 hours after substance ingestion to assess the concentration of inflammatory biomarkers, together with administration of the Montgomery-Åsberg Depression Rating Scale.

Results

At pre-treatment, patients showed higher C-reactive protein levels than healthy controls and a significant negative correlation between C-reactive protein and serum cortisol levels was revealed ( rho = –0.40, n = 14). C-reactive protein in those patients was not correlated with Montgomery-Åsberg Depression Rating Scale scores. We observed a significant reduction of C-reactive protein levels across time in both patients and controls treated with ayahuasca, but not with placebo. Patients treated with ayahuasca showed a significant correlation ( rho = + 0.57) between larger reductions of C-reactive protein and lower depressive symptoms at 48 hours after substance ingestion (Montgomery-Åsberg Depression Rating Scale). No significant result with respect to interleukin 6 and brain-derived neurotrophic factor was found. Furthermore, these biomarkers did not predict the antidepressant response or remission rates observed.

Conclusions

These findings enhance the understanding of the biological mechanisms behind the observed antidepressant effects of ayahuasca and encourage further clinical trials in adults with depression.

Available with Blossom Pro

Research Summary of 'Changes in inflammatory biomarkers are related to the antidepressant effects of Ayahuasca'

Introduction

Major depressive disorder (MDD) is heterogeneous and many patients do not achieve sustained remission with standard pharmacological treatments, leaving a subset with treatment-resistant depression. Earlier research has identified raised circulating inflammatory markers in some people with depression, notably interleukin 6 (IL-6) and C-reactive protein (CRP), and has linked chronic stress, reduced brain-derived neurotrophic factor (BDNF), and inflammation to depressive pathophysiology. Findings on whether conventional antidepressants reduce inflammatory markers are inconsistent, and baseline inflammation may influence treatment effects. Classical serotonergic psychedelics have attracted renewed interest as potentially fast-acting antidepressants, and some preclinical and mechanistic work has proposed direct anti-inflammatory actions mediated via 5-HT2A and sigma-1 (σ1R) receptors. Leite Galvão‑Coelho and colleagues set out to examine whether a single dose of ayahuasca alters blood inflammatory biomarkers and whether such changes relate to antidepressant effects. Using data from a randomised, double-blind, placebo-controlled trial in adults with treatment-resistant depression and healthy controls, the investigators measured plasma CRP and serum IL-6 before treatment and 48 hours after dosing, and tested correlations with serum cortisol and BDNF as well as associations with clinical change on the Montgomery-Åsberg Depression Rating Scale (MADRS). They hypothesised that changes in inflammatory markers would correlate with symptom improvement and might predict antidepressant response or remission at 48 hours.

Expert Research Summaries

Go Pro to access AI-powered section-by-section summaries, editorial takes, and the full research toolkit.

Full Text PDF

Full Paper PDF

Create a free account to open full-text PDFs.

Study Details

References (10)

Papers cited by this study that are also in Blossom

Modulation of Serum Brain-Derived Neurotrophic Factor by a Single Dose of Ayahuasca: Observation From a Randomized Controlled Trial

Galvão-Coelho, N. L., de Almeida, R. N., de Menezes Galvão, A. C. et al. · Frontiers in Psychology (2019)

The hallucinogenic world of tryptamines: an updated review

Araújo, A. M., Carvalho, F. M., Carvalho, M. et al. · Archives of Toxicology (2015)

Seeing with the eyes shut: Neural basis of enhanced imagery following ayahuasca ingestion

De Araujo, D. B., Ribeiro, S., Cecchi, G. A. et al. · Human Brain Mapping (2011)

Psychedelics as anti-inflammatory agents

Flanagan, T. W., Nichols, C. D. · International Review of Psychiatry (2018)

Antidepressant effects of a single dose of ayahuasca in patients with recurrent depression: a preliminary report

Osório, F. L., Sanches, R. F., Macedo, L. et al. · brazilian Journal of Psychiatry (2015)

420 cited
The psychedelic state induced by ayahuasca modulates the activity and connectivity of the default mode network

Palhano-Fontes, F., Andrade, K. C., Tófoli, L.F. et al. · PLOS ONE (2015)

Antidepressant effects of a single dose of ayahuasca in patients with recurrent depression a SPECT study

Sanches, R. F., Osório, F. L., Dos Santos, R. G. et al. · Journal of Clinical Psychopharmacology (2016)

380 cited

Cited By (21)

Papers in Blossom that reference this study

Psychedelic therapy and postpartum depression: priorities and prospects

Thuery, G., Crossen, F., Mc Loone, D. et al. · Therapeutic Advances in Psychopharmacology (2026)

Pilot study of psilocybin in patients with post-treatment lyme disease

Garcia-Romeu, A., Naudé, G. P., Rebman, A. W. et al. · Scientific Reports (2026)

Predicting and exploring ayahuasca effects: Perception, mind-wandering, and EEG oscillations

Silva-Costa, N., Pessoa, J. A., Andrade, K. C. et al. · Journal of Psychopharmacology (2025)

Mind over matter: the microbial mindscapes of psychedelics and the gut-brain axis

Caspani, G., Ruffell, S. G. D., Tsang, WF. et al. · Pharmacological Research (2024)

Psilocybin induces acute and persisting alterations in immune status in healthy volunteers: An experimental, placebo-controlled study

Mason, N. L., Szabo, A., Kuypers, K. P. C. et al. · Brain Behavior and Immunity - Health (2023)

Subacute effects of a single dose of psilocybin on biomarkers of inflammation in healthy humans: An open-label preliminary investigation

Burmester, D., Madsen, M. K., Szabo, A. et al. · Comprehensive Psychoneuroendocrinology (2023)

Prophylactic action of ayahuasca in a non-human primate model of depressive-like behavior

Galvão-Coelho, N. L., De Meiroz Grilo, M. L. P., de Sousa, G. M. et al. · Frontiers in Behavioural Neuroscience (2022)

Ayahuasca, a psychedelic beverage, modulates neuroplasticity induced by ethanol in mice

Almeida, C. A. F., Pereira, B. P., Pereira Junior, A. A. et al. · Behavioural Brain Research (2022)

Psychedelic Therapy's Transdiagnostic Effects: A Research Domain Criteria (RDoC) Perspective

Dursun, S. M., Kelly, J. R., Gillan, C. M. et al. · Frontiers in Psychiatry (2021)

Show all 21 papers
Psychedelics as Novel Therapeutics in Alzheimer’s Disease: Rationale and Potential Mechanisms

Garcia-Romeu, A., Darcy, S., Jackson, H. et al. · Current Topics in Behavioral Neurosciences (2021)

Improving cognitive functioning in major depressive disorder with psychedelics: a dimensional approach

Kuiperes, Z., Schreiber, R. · Neurobiology of Learning and Memory (2021)

Protocol for Outcome Evaluation of Ayahuasca-Assisted Addiction Treatment: The Case of Takiwasi Center

Rush, B., Marcus, O., García, S. et al. · Frontiers in Pharmacology (2021)

19 cited
37 cited
Hallucinogenic/psychedelic 5HT2A receptor agonists as rapid antidepressant therapeutics: Evidence and mechanisms of action

Dos Santos, R. G., Hallak, J. E., Baker, G. et al. · Journal of Psychopharmacology (2021)

Anti-inflammatory activity of ayahuasca and its implications for the treatment of neurological and psychiatric diseases

da Silva, M. G., Daros, G. C., de Bitencourt, R. M. · Behavioural Brain Research (2021)

15 cited
The History of Psychedelics in Psychiatry

Nichols, D. E., Walter, H. · Pharmacopsychiatry (2020)

The ritual use of ayahuasca during treatment of severe physical illnesses: a qualitative study

Maia, L. O., Daldegan-Bueno, D., Tófoli, L.F. · Journal of Psychoactive Drugs (2020)

21 cited
Ketamine and Serotonergic Psychedelics: Common Mechanisms Underlying the Effects of Rapid-Acting Antidepressants

Kadriu, B., Greenwald, M., Ba et al. · International Journal of Neuropsychopharmacology (2020)

Psychedelics as a Novel Approach to Treating Autoimmune Conditions

Thompson, C., Szabo, A. · Immunology Letters (2020)

Your Personal Research Library

Go Pro to save papers, add notes, rate studies, and organize your research into custom shelves.